Activity and Resistance Assessment of a New OSBP Inhibitor, R034-1, in Phytophthora capsici and the Detection of Point Mutations in PcORP1 that Confer Resistance.
Identifieur interne : 000309 ( Main/Exploration ); précédent : 000308; suivant : 000310Activity and Resistance Assessment of a New OSBP Inhibitor, R034-1, in Phytophthora capsici and the Detection of Point Mutations in PcORP1 that Confer Resistance.
Auteurs : Dong Lin [République populaire de Chine] ; Zhaolin Xue [République populaire de Chine] ; Jianqiang Miao [République populaire de Chine] ; Zhongqiao Huang [République populaire de Chine] ; Xili Liu [République populaire de Chine]Source :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2020.
Abstract
R034-1 is a new member of the piperidinyl thiazole isoxazoline class of fungicides that shows high activity against most plant-pathogenic oomycetes and could effectively inhibit several developmental stages of Phytophthora capsici. Here, the potential resistance risk for R034-1 was evaluated in P. capsici. The baseline sensitivities of 135 isolates to R034-1 showed a unimodal curve, with a mean EC50 value of 0.004 μg/mL. Twelve resistant mutants were generated by fungicide adaptation and displayed lower fitness compared to parental isolates, which suggests that the resistance risk of P. capsici to R034-1 is low. R034-1 and oxathiapiprolin are structurally related, and resistant isolates display cross-resistance to both compounds, suggesting that these fungicides may target the same oxysterol binding protein. Comparison of PcORP1 genes in the resistant mutants and their parental isolates revealed (N767S, N767I, and G700V) amino acid substitutions in the R034-1 resistant mutant. Causality was functionally validated using site-directed mutagenesis of the target gene using the CRISPR/Cas9 system.
DOI: 10.1021/acs.jafc.0c05531
PubMed: 33191734
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Activity and Resistance Assessment of a New OSBP Inhibitor, R034-1, in <i>Phytophthora capsici</i> and the Detection of Point Mutations in PcORP1 that Confer Resistance.</title><author><name sortKey="Lin, Dong" sort="Lin, Dong" uniqKey="Lin D" first="Dong" last="Lin">Dong Lin</name><affiliation wicri:level="1"><nlm:affiliation>China Agricultural University, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>China Agricultural University, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xue, Zhaolin" sort="Xue, Zhaolin" uniqKey="Xue Z" first="Zhaolin" last="Xue">Zhaolin Xue</name><affiliation wicri:level="1"><nlm:affiliation>China Agricultural University, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>China Agricultural University, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Miao, Jianqiang" sort="Miao, Jianqiang" uniqKey="Miao J" first="Jianqiang" last="Miao">Jianqiang Miao</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712110, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712110</wicri:regionArea><wicri:noRegion>Yangling 712110</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Zhongqiao" sort="Huang, Zhongqiao" uniqKey="Huang Z" first="Zhongqiao" last="Huang">Zhongqiao Huang</name><affiliation wicri:level="1"><nlm:affiliation>China Agricultural University, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>China Agricultural University, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Xili" sort="Liu, Xili" uniqKey="Liu X" first="Xili" last="Liu">Xili Liu</name><affiliation wicri:level="1"><nlm:affiliation>China Agricultural University, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>China Agricultural University, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712110, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712110</wicri:regionArea><wicri:noRegion>Yangling 712110</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33191734</idno><idno type="pmid">33191734</idno><idno type="doi">10.1021/acs.jafc.0c05531</idno><idno type="wicri:Area/Main/Corpus">000006</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000006</idno><idno type="wicri:Area/Main/Curation">000006</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000006</idno><idno type="wicri:Area/Main/Exploration">000006</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Activity and Resistance Assessment of a New OSBP Inhibitor, R034-1, in <i>Phytophthora capsici</i> and the Detection of Point Mutations in PcORP1 that Confer Resistance.</title><author><name sortKey="Lin, Dong" sort="Lin, Dong" uniqKey="Lin D" first="Dong" last="Lin">Dong Lin</name><affiliation wicri:level="1"><nlm:affiliation>China Agricultural University, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>China Agricultural University, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xue, Zhaolin" sort="Xue, Zhaolin" uniqKey="Xue Z" first="Zhaolin" last="Xue">Zhaolin Xue</name><affiliation wicri:level="1"><nlm:affiliation>China Agricultural University, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>China Agricultural University, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Miao, Jianqiang" sort="Miao, Jianqiang" uniqKey="Miao J" first="Jianqiang" last="Miao">Jianqiang Miao</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712110, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712110</wicri:regionArea><wicri:noRegion>Yangling 712110</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Zhongqiao" sort="Huang, Zhongqiao" uniqKey="Huang Z" first="Zhongqiao" last="Huang">Zhongqiao Huang</name><affiliation wicri:level="1"><nlm:affiliation>China Agricultural University, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>China Agricultural University, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Xili" sort="Liu, Xili" uniqKey="Liu X" first="Xili" last="Liu">Xili Liu</name><affiliation wicri:level="1"><nlm:affiliation>China Agricultural University, Beijing 100193, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>China Agricultural University, Beijing 100193</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712110, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712110</wicri:regionArea><wicri:noRegion>Yangling 712110</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="eISSN">1520-5118</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">R034-1 is a new member of the piperidinyl thiazole isoxazoline class of fungicides that shows high activity against most plant-pathogenic oomycetes and could effectively inhibit several developmental stages of <i>Phytophthora capsici</i>. Here, the potential resistance risk for R034-1 was evaluated in <i>P. capsici</i>. The baseline sensitivities of 135 isolates to R034-1 showed a unimodal curve, with a mean EC<sub>50</sub> value of 0.004 μg/mL. Twelve resistant mutants were generated by fungicide adaptation and displayed lower fitness compared to parental isolates, which suggests that the resistance risk of <i>P. capsici</i> to R034-1 is low. R034-1 and oxathiapiprolin are structurally related, and resistant isolates display cross-resistance to both compounds, suggesting that these fungicides may target the same oxysterol binding protein. Comparison of <i>PcORP1</i> genes in the resistant mutants and their parental isolates revealed (N767S, N767I, and G700V) amino acid substitutions in the R034-1 resistant mutant. Causality was functionally validated using site-directed mutagenesis of the target gene using the CRISPR/Cas9 system.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33191734</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-5118</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Nov</Month><Day>16</Day></PubDate></JournalIssue><Title>Journal of agricultural and food chemistry</Title><ISOAbbreviation>J Agric Food Chem</ISOAbbreviation></Journal><ArticleTitle>Activity and Resistance Assessment of a New OSBP Inhibitor, R034-1, in <i>Phytophthora capsici</i> and the Detection of Point Mutations in PcORP1 that Confer Resistance.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.jafc.0c05531</ELocationID><Abstract><AbstractText>R034-1 is a new member of the piperidinyl thiazole isoxazoline class of fungicides that shows high activity against most plant-pathogenic oomycetes and could effectively inhibit several developmental stages of <i>Phytophthora capsici</i>. Here, the potential resistance risk for R034-1 was evaluated in <i>P. capsici</i>. The baseline sensitivities of 135 isolates to R034-1 showed a unimodal curve, with a mean EC<sub>50</sub> value of 0.004 μg/mL. Twelve resistant mutants were generated by fungicide adaptation and displayed lower fitness compared to parental isolates, which suggests that the resistance risk of <i>P. capsici</i> to R034-1 is low. R034-1 and oxathiapiprolin are structurally related, and resistant isolates display cross-resistance to both compounds, suggesting that these fungicides may target the same oxysterol binding protein. Comparison of <i>PcORP1</i> genes in the resistant mutants and their parental isolates revealed (N767S, N767I, and G700V) amino acid substitutions in the R034-1 resistant mutant. Causality was functionally validated using site-directed mutagenesis of the target gene using the CRISPR/Cas9 system.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Dong</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>China Agricultural University, Beijing 100193, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xue</LastName><ForeName>Zhaolin</ForeName><Initials>Z</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-2469-7968</Identifier><AffiliationInfo><Affiliation>China Agricultural University, Beijing 100193, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miao</LastName><ForeName>Jianqiang</ForeName><Initials>J</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-4683-6933</Identifier><AffiliationInfo><Affiliation>State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712110, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Zhongqiao</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>China Agricultural University, Beijing 100193, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Xili</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>China Agricultural University, Beijing 100193, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712110, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Agric Food Chem</MedlineTA><NlmUniqueID>0374755</NlmUniqueID><ISSNLinking>0021-8561</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Phytophthora capsici</Keyword><Keyword MajorTopicYN="N">R034-1</Keyword><Keyword MajorTopicYN="N">high activity</Keyword><Keyword MajorTopicYN="N">point mutation</Keyword><Keyword MajorTopicYN="N">resistance assessment</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>16</Day><Hour>8</Hour><Minute>41</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33191734</ArticleId><ArticleId IdType="doi">10.1021/acs.jafc.0c05531</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Lin, Dong" sort="Lin, Dong" uniqKey="Lin D" first="Dong" last="Lin">Dong Lin</name></noRegion><name sortKey="Huang, Zhongqiao" sort="Huang, Zhongqiao" uniqKey="Huang Z" first="Zhongqiao" last="Huang">Zhongqiao Huang</name><name sortKey="Liu, Xili" sort="Liu, Xili" uniqKey="Liu X" first="Xili" last="Liu">Xili Liu</name><name sortKey="Liu, Xili" sort="Liu, Xili" uniqKey="Liu X" first="Xili" last="Liu">Xili Liu</name><name sortKey="Miao, Jianqiang" sort="Miao, Jianqiang" uniqKey="Miao J" first="Jianqiang" last="Miao">Jianqiang Miao</name><name sortKey="Xue, Zhaolin" sort="Xue, Zhaolin" uniqKey="Xue Z" first="Zhaolin" last="Xue">Zhaolin Xue</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000309 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000309 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:33191734
|texte= Activity and Resistance Assessment of a New OSBP Inhibitor, R034-1, in Phytophthora capsici and the Detection of Point Mutations in PcORP1 that Confer Resistance.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:33191734" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |